M-Scan has unrivalled expertise in biopharmaceutical analysis. As originators of MS Peptide Mapping, together with other mass spectrometry strategies related to protein/glycoprotein characterisation, our customers benefit from our vast experience gained from analysing hundreds of biotechnology products, including antibodies.
M-Scan provides individual analyses, or a full antibody analysis package, tailored to the FDA and ICH guidelines and available to GLP/GMP, including:
- Amino acid sequence analysis, using automated sequencing via Edman degradation and/or MS/MS sequence analysis
- Amino acid composition analysis to provide protein concentration, extinction coefficient and/or molar amino acid ratios
- N-Terminal and C-Terminal amino acid sequence analysis, using automated Edman chemistry and/or mass-spectrometric mapping/sequencing strategies
- Peptide MS Mapping analysis, using digestion and MS strategies
- Disulphide bridge analysis, using MS and MS/MS
- Carbohydrate structure, including monosaccharide composition analysis, oligosaccharide population screening by HPAEC-PAD and MS, linkage analysis and glycosylation site determination
- Determination of intact molecular weight
- Electrophoretic and isoform patterns, including CE
- Liquid chromatographic patterns, including RP-HPLC, SEC and IEX
- Identification of post-translational modifications
- Spectroscopic profiling, using CD
- Identification of product related impurities, including degradation
products (deamidation, oxidation, mis-matched S-S bridge forms) - NMR and AUC are available at research level
M-Scan Brochure: Biotechnology and Pharmaceutical Analysis (8pp.)